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Journal / Neuroscience / Ændrede Roller Tube metode for netop lokaliseret…
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Neuroscience

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JoVE Journal Neuroscience
Ændrede Roller Tube metode for netop lokaliseret og gentagne intermitterende Imaging under langtidskulturer af hjernen skiver i et lukket System
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Ændrede Roller Tube metode for netop lokaliseret og gentagne intermitterende Imaging under langtidskulturer af hjernen skiver i et lukket System

Article DOI: 10.3791/56436 • 09:52 min • December 28th, 2017
December 28th, 2017 •
*1, *1, *1, 1, 1,2, 1, 1,3, 1, 1
1Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program, Colorado State University, 2IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS, Universidade do Porto, 3Denali Therapeutics
* These authors contributed equally

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Præsenteret her er en modificeret roller tube metode til dyrkning og intermitterende høj opløsning billeddannelse af hjernen, gnaver skiver i mange uger med præcise repositionering på photoetched coverslips. Neuronal levedygtighed og skive morfologi er godt vedligeholdt. Anvendelser af denne fuldstændig lukket system ved hjælp af virus til celletype specifikke udtryk er fastsat.

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Neurovidenskab spørgsmålet 130 gnaver hippocampus viral-medieret genekspression Konfokal mikroskopi photoetched coverslips neurodegenerative sygdomme cofilin patologi Alzheimers sygdom
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