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Focal Macropatch optagelser af Synaptic strømninger fra Drosophila larve neuromuskulære Junction
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Neuroscience
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JoVE Journal Neuroscience
Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

Focal Macropatch optagelser af Synaptic strømninger fra Drosophila larve neuromuskulære Junction

DOI:
10.3791/56493-v

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07:01 min

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September 25, 2017

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,
1Department of Neurology, School of Medicine,Wayne State University, 2Department of Anatomy and Cell Biology, School of Medicine,Wayne State University

Chapters

  • 00:05Title
  • 00:39Fabrication of Recording Electrodes
  • 01:53Additional Preparation
  • 03:17EJC Electrical Recording
  • 04:33EJC Electrical Stimulation and Seal Resistance
  • 05:34Results: Accurate Detection of Release Events
  • 06:29Conclusion

Summary

Automatic Translation

Automatic Translation

Synaptic strømme kan registreres focally fra visualiseret synaptic boutons i Drosophila tredje instar larver neuromuskulære junction. Denne teknik gør det muligt for overvågning af aktiviteten af et enkelt synaptic bouton.

Tags

DrosophilaLarval Neuromuscular JunctionSynaptic CurrentsFocal Macropatch RecordingsSynaptic BoutonsSynaptic TransmissionSynaptic ProteinsMicroelectrodeHemolymph-like SolutionHL3 SolutionStimulation PipettesDissection PlatesWandering Third Instar LarvaeFiletingPinningNerve Cutting

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