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电生理测量在中性转运体活性研究中的应用

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Biology
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JoVE Journal Biology

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

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电生理测量在中性转运体活性研究中的应用

DOI:

10.3791/56630-v

•

11:51 min

•

February 03, 2018

•

Victor Babich², Matthew K. Henry, Francesca Di Sole

¹Physiology and Pharmacology Department,Des Moines University, ²School of Liberal Arts and Sciences,Mercy College of Health Sciences

Chapters

00:00Title
02:47Preparation of Pipettes of ISE
04:02Preparation of Patch-clamp Pipette
05:40Preparation of Cells
05:59Recording of Transporter Activity
10:01Representative Results
11:19Conclusion

Summary

Automatic Translation

本文介绍了质子选择性电极和膜片夹紧方法在质子传输系统中的应用。这些方法克服了一般用于研究质子转运活动的一些技术限制, 如灵敏度适中、时间分辨力和细胞内环境控制不足。

Tags

Electrophysiology Ion-selective Microelectrodes Sodium-hydrogen Exchanger NHE Proton Flux PH Regulation Whole Cell Patch Clamping Proton-selective Microelectrode Epithelium Ion Transport Electro-neutral Transporters

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