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电生理测量在中性转运体活性研究中的应用
JoVE Journal
Biology
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JoVE Journal Biology
Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
DOI:

11:51 min

February 03, 2018

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Chapters

  • 00:00Title
  • 02:47Preparation of Pipettes of ISE
  • 04:02Preparation of Patch-clamp Pipette
  • 05:40Preparation of Cells
  • 05:59Recording of Transporter Activity
  • 10:01Representative Results
  • 11:19Conclusion

Summary

Automatic Translation

本文介绍了质子选择性电极和膜片夹紧方法在质子传输系统中的应用。这些方法克服了一般用于研究质子转运活动的一些技术限制, 如灵敏度适中、时间分辨力和细胞内环境控制不足。

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