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Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection
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Neuroscience
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JoVE Journal Neuroscience
Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

DOI:
10.3791/56757-v

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09:23 min

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March 09, 2018

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, ,
1Department of Anatomy, Chicago College of Osteopathic Medicine (CCOM),Midwestern University, 2Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine (CCOM),Midwestern University

Chapters

  • 00:04Title
  • 00:55Harvesting DRG from Mouse Embryos
  • 03:59Isolating Single Neurons from DRG and HSV-1 Infection
  • 06:07Immunofluorescence
  • 07:11Results: Investigation of HSV-1 Entry Model in DRG-derived Dissociated Cells
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

In this report, the advantages of organotypic cultures and dissociated primary cultures of mouse-derived dorsal root ganglia are highlighted to investigate a wide range of mechanisms associated with neuron-glial interaction, neuroplasticity, neuroinflammation, and response to viral infection.

Tags

Keywords: Dorsal Root GangliaDRGExplantDissociated Cell ModelNeuroplasticityNeuroinflammationViral InfectionSpinal CordPeripheral NerveIntervertebral ForamenPrimary Cell Culture

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