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成年小鼠背根节外植体和分离细胞模型调查可塑性和对包括病毒感染在内的环境侮辱的反应
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection
DOI:

09:23 min

March 09, 2018

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Chapters

  • 00:04Title
  • 00:55Harvesting DRG from Mouse Embryos
  • 03:59Isolating Single Neurons from DRG and HSV-1 Infection
  • 06:07Immunofluorescence
  • 07:11Results: Investigation of HSV-1 Entry Model in DRG-derived Dissociated Cells
  • 08:06Conclusion

Summary

Automatic Translation

在本报告中, 强调了脊髓培养和分离的原代培养的小鼠背根神经节的优势, 以调查与神经元-胶质细胞相互作用的广泛机制, 可塑性,neuroinflammation 和对病毒感染的反应。

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