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成年小鼠背根节外植体和分离细胞模型调查可塑性和对包括病毒感染在内的环境侮辱的反应
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection
Please note that all translations are automatically generated.
Click here for the English version.
成年小鼠背根节外植体和分离细胞模型调查可塑性和对包括病毒感染在内的环境侮辱的反应
DOI:
10.3791/56757-v
•
09:23 min
•
March 09, 2018
•
Michele Fornaro
,
Harsh Sharthiya
,
Vaibhav Tiwari
1
Department of Anatomy, Chicago College of Osteopathic Medicine (CCOM)
,
Midwestern University
,
2
Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine (CCOM)
,
Midwestern University
Chapters
00:04
Title
00:55
Harvesting DRG from Mouse Embryos
03:59
Isolating Single Neurons from DRG and HSV-1 Infection
06:07
Immunofluorescence
07:11
Results: Investigation of HSV-1 Entry Model in DRG-derived Dissociated Cells
08:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在本报告中, 强调了脊髓培养和分离的原代培养的小鼠背根神经节的优势, 以调查与神经元-胶质细胞相互作用的广泛机制, 可塑性,neuroinflammation 和对病毒感染的反应。
Tags
Dorsal Root Ganglia
DRG
Explant
Dissociated Cell Model
Neuroplasticity
Neuroinflammation
Viral Infection
Spinal Cord
Peripheral Nerve
Intervertebral Foramen
Primary Cell Culture
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