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Journal
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Immunology and Infection
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ジカ特定診断エピトープの探索
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Zika Virus Specific Diagnostic Epitope Discovery
Please note that all translations are automatically generated.
Click here for the English version.
ジカ特定診断エピトープの探索
DOI:
10.3791/56784-v
•
11:37 min
•
December 12, 2017
•
Maite Sabalza
,
Cheryl A. Barber
,
William R. Abrams
,
Richard Montagna
,
Daniel Malamud
3
1
Department of Basic Sciences and Craniofacial Development
,
New York University College of Dentistry
,
2
Rheonix, Inc.
,
3
Department of Medicine
,
New York University Langone School of Medicine
Chapters
00:05
Title
01:21
Installing the ZIKV High-density Peptide Microarray Slide in a Specific Incubation Tray
02:38
Background Interation Detection: Staining the Microarray with Secondary Antibodies
04:59
Exposure of the Microarray to Host Serum
06:08
Staining with Secondary Antibodies and Labeled Control Antibodies
07:12
Microarray Scanning, Anaysis, and Storage
09:10
Results: Peptide Microarray Scanned Images and Representative Zika Virus Epitope Consensus Sequence Reacting with IgM on the Host Serum Sample
10:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルでは高密度ペプチド マイクロ アレイを用いたジカ ウイルス特定診断ペプチドを発見する手法について述べる。このプロトコルは、他の新興感染症に立ち向かえない適合できます。
Tags
Zika Virus
Diagnostic Assay
Antibodies
IgM
IgG
Microarray
Proteome
Epitopes
Serum
Pathogens
Protein Sequence
Incubation Tray
Glass Slide
Seal
Blocking Buffer
Secondary Antibody
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