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检测暴露于有机氯农药的肝细胞线粒体功能的实验方案
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Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides
Please note that all translations are automatically generated.
Click here for the English version.
检测暴露于有机氯农药的肝细胞线粒体功能的实验方案
DOI:
10.3791/56800-v
•
08:39 min
•
September 16, 2020
•
Qian Liu
2,3
,
Zhaoyan Jiang
,
Aihua Gu
2
1
State Key Laboratory of Reproductive Medicine, Institute of Toxicology
,
Nanjing Medical University
,
2
Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health
,
Nanjing Medical University
,
3
Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease
,
Tongji University School of Medicine
Chapters
00:04
Title
00:36
Mitochondrial Fluorescence Intensity and Cellular ATP Detection
02:45
Mitochondrial Membrane Potential Measurements
04:39
Oxygen Consumption Rate (OCR) Measurements
06:22
Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
07:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
了解环境有机氯农药(OCPs)对肝细胞线粒体功能的影响,对于探讨OCP引起代谢紊乱的机理十分重要。本文提出了检测肝线粒体功能的详细方法。
Tags
Mitochondrial Function
Hepatocytes
Organochlorine Pesticides
ATP Level
Oxygen Consumption
HepG2 Cells
Beta-hexachlorcyclohexane
Mitochondrial Green Fluorescence Probe
Confocal Microscopy
Cellular ATP
Luminometer
Mitochondrial Membrane Potential
JC1 Dye
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