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Protocolo Experimental para Detecção de Função Mitocondrial em Hepatócitos Expostos a Pesticidas Organoclorine
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1State Key Laboratory of Reproductive Medicine, Institute of Toxicology,Nanjing Medical University, 2Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health,Nanjing Medical University, 3Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease,Tongji University School of Medicine
Chapters
- 00:04Title
- 00:36Mitochondrial Fluorescence Intensity and Cellular ATP Detection
- 02:45Mitochondrial Membrane Potential Measurements
- 04:39Oxygen Consumption Rate (OCR) Measurements
- 06:22Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
- 07:43Conclusion
Compreender a influência dos pesticidas organocloritos ambientais (OCPs) na função mitocondrial nos hepatócitos é importante na exploração do mecanismo de OCPs causando distúrbios metabólicos. Este artigo apresenta métodos detalhados na detecção da função mitôndrial hepática.
