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Strømlinet 3D cerebellare differentiering protokol med valgfri 2D ændring
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
DOI:

10:15 min

December 09, 2017

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Chapters

  • 00:05Title
  • 00:443D “Organoid” Differentiation
  • 04:32Alternative 2D Differentiation Culture
  • 08:28Results: 3D Products Exhibit Markers and Structures of Early Neuroepithelium, and 2D Products Exhibit Cerebellar Markers and Functional Neuronal Activity
  • 09:48Conclusion

Summary

Automatic Translation

Vi beskriver et forenklet 3D differentiering protokol for hPSCs, bruger defineret medium og reduceret vækstfaktorer, frembringe celle aggregater med tidlige neuroepithelial strukturer og positiv for cerebellare-associerede markører, samt en valgfri 2D modifikation for differentiere celler som en éncellelag til at generere funktionelle neuroner.

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