/
/
Måling af Deformability og roede blodlegemer heterogenitet i blodet ved Ektacytometry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Pediatrics,Saint Louis University School of Medicine, 2Molecular and Clinical Nutrition Section, Digestive Diseases Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 3Molecular and Clinical Hematology Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 4Sickle Cell Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 5Edward A. Doisy Department of Biochemistry and Molecular Biology,Saint Louis University School of Medicine, 6Red Cell Physiology Laboratory,New York Blood Center
Chapters
- 00:05Title
- 01:05Ektacytometer Set-up
- 01:48Measuring Deformability as a Function of Increasing Shear Stress
- 04:10Measuring Cellular Heterogeneity
- 05:20Osmotic Gradient Ektacytometry
- 07:16Results: Diffraction Pattern Distortion and Relationship to Hemoglobin
- 08:41Conclusion
Her præsenterer vi teknikker til at måle roede blodlegemer deformability og cellulære heterogenitet af ektacytometry. Disse teknikker anvendes til generelle undersøgelser af roede blodlegemer deformability og specifikke undersøgelser af blodsygdomme karakteriseret ved tilstedeværelsen af både stive og deformerbare røde blodlegemer i cirkulation, såsom seglcelleanæmi.
