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整合酶缺陷慢病毒载体载体的制备及其在细胞 CRISPR/Cas9-mediated 基因敲除中的表达

整合酶缺陷慢病毒载体载体的制备及其在细胞 CRISPR/Cas9-mediated 基因敲除中的表达

Article doi: 10.3791/56915
December 12th, 2017

Summary December 12th, 2017

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我们描述了整合酶缺乏慢病毒载体载体 (IDLVs) 作为向细胞传递 CRISPR/Cas9 的工具的生产策略。由于能够在细胞中调节快速和健壮的基因编辑, IDLVs 为基因的传递提供了一个更安全、同样有效的载体平台, 与整合酶能力的载体相比。

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