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Metoder for iscenesættelse puppe perioder og måling af Wing pigmentering af Drosophila guttifera
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JoVE Journal Biology
Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera

Metoder for iscenesættelse puppe perioder og måling af Wing pigmentering af Drosophila guttifera

DOI:
10.3791/56935-v

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08:03 min

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January 24, 2018

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, , ,
1Graduate School of Science,Kyoto University, 2Graduate School of Science,Osaka City University, 3The Hakubi Center for Advanced Research,Kyoto University, 4Graduate School of Environmental Science,Hokkaido University

Chapters

  • 00:05Title
  • 00:46Puparium Removal
  • 02:13Image Preparation
  • 03:55Definition of Point A
  • 06:18Measurements
  • 07:19Results: Pigmentation Timing Identified
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

Protokoller for iscenesættelse puppe perioder og måling af wing pigmentering af Drosophila guttifera er beskrevet. Iscenesættelse og kvantificering af pigmentering giver et solidt grundlag for at studere udviklingsmæssige mekanismer af voksne træk og aktiverer artskrydsede sammenligning af trait udvikling.

Tags

Keywords: Drosophila GuttiferaPupal PeriodWing PigmentationPuparium RemovalForcepsPhosphate-buffered SalineAnesthetizationWing Measurement

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