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Chapters
- 00:11Introduction
- 04:44Odor dilution
- 08:20Preparation of larvae for tracking
- 10:38Preparation of plates for tracking
- 12:20Creation of odor gradient
- 17:07Tracking system
- 17:28Behavioral assay
- 20:24Single source assay
- 22:31Test of smell in individual larvae
- 23:33Multiple source assay
- 27:34Future directions
In this video article, we describe a new method allowing the construction of odorant gradients with stable and controllable geometries. We briefly illustrate how these gradients can be used to screen for olfactory defects (full and partial anosmia) and to study more subtle features of chemotaxis behavior.
Tags
High-resolution MeasurementOdor-driven BehaviorDrosophila LarvaeOlfactory ResponsesPetri DishesPeripheral Odor SourceDiffusion Of Odorant MoleculesStable GradientQuantitative CorrelationSensory InputsBehavioral ResponsesOdorant Stimulus ConditionsVideo ArticleConstruction Of Odorant GradientsStable And Controllable GeometriesScreen For Olfactory DefectsAnosmiaChemotaxis Behavior
