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Forberede frisk Retinal skiver fra voksne sebrafisk for Ex Vivo Imaging eksperimenter
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Forberede frisk Retinal skiver fra voksne sebrafisk for Ex Vivo Imaging eksperimenter

Article DOI: 10.3791/56977-v • 11:01 min • May 9th, 2018
May 9th, 2018 •
1, 1, 1,2, 1,2
1Department of Biochemistry, University of Washington, 2Department of Ophthalmology, University of Washington

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Imaging Netthinnen vev kan gi encellede informasjon som ikke kan samles inn fra tradisjonelle biokjemiske metoder. Denne protokollen beskriver utarbeidelse av netthinnen skiver fra sebrafisk for AC confocal bildebehandling. Fluorescerende kodet genetisk sensorer eller indikator fargestoffer tillater visualisering av mange biologiske prosesser i forskjellige retinal celletyper.

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Nevrovitenskap problemet 135 sebrafisk netthinnen photoreceptor vev slice fluorescens biosensor live bildebehandling mikroskopi nevrovitenskap molekylærbiologi cellebiologi
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