9,108 Views
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11:01 min
May 09, 2018
DOI:
10.3791/56977-v
網膜組織を撮像すると、従来の生化学的方法から収集することはできません単一セル情報が提供できます。このプロトコルでは、共焦点レーザー顕微鏡のゼブラフィッシュの網膜スライスの準備について説明します。蛍光遺伝子センサーをエンコードまたはインジケーター染料網膜細胞の多数の生物学的過程の可視化を許可します。
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Cite this Article
Giarmarco, M. M., Cleghorn, W. M., Hurley, J. B., Brockerhoff, S. E. Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments. J. Vis. Exp. (135), e56977, doi:10.3791/56977 (2018).
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