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从成年斑马鱼中制备新鲜视网膜切片用于体成像实验
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
DOI:

11:01 min

May 09, 2018

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Chapters

  • 00:04Title
  • 00:44Preparing Animals and Equipment
  • 02:55Dissecting the Retina
  • 04:36Flat-mounting the Retina
  • 06:21Slicing the Retina and Assembling the Imaging Ladder
  • 08:39Results: Visualizing Ca2-dynamics in Cone Photoreceptors
  • 10:19Conclusion

Summary

Automatic Translation

影像视网膜组织可以提供无法从传统生化方法收集的单细胞信息。该协议描述了从斑马鱼的视网膜切片的制备共焦成像。荧光基因编码的传感器或指示染料允许可视化许多生物学过程中的不同的视网膜细胞类型。

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