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Journal
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Immunology and Infection
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以层流为基础的检测培养血管细胞和粘附血小板白细胞的研究
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
Please note that all translations are automatically generated.
Click here for the English version.
以层流为基础的检测培养血管细胞和粘附血小板白细胞的研究
DOI:
10.3791/57009-v
•
08:50 min
•
April 09, 2018
•
Tanja Vajen
,
Alexandra C.A. Heinzmann
,
Annemiek Dickhout
,
Zhen Zhao
,
Magdolna Nagy
,
Johan W.M. Heemskerk
,
Rory R. Koenen
1
Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM)
,
Maastricht University
,
2
Department of Vascular Surgery, Shanghai Ninth People’s Hospital
,
Shanghai Jiao Tong University, School of Medicine
Chapters
00:05
Title
00:57
Flow-based Assay with Human Cells
02:28
Preparation of Adherent Platelet and Vascular Cell Monolayers
03:56
Fluorescent Labeling and Perfusion of Leukocytes
06:32
Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
08:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在血管壁损伤或炎症期间, 白细胞与血管细胞和血小板相互作用。在这里, 我们描述了一种简单的基于层流的检测方法来表征白细胞与其细胞伙伴之间相互作用的分子机制。
Tags
Keyword Extraction:Leukocyte Recruitment
Adherent Vascular Cells
Adherent Platelets
Flow Conditions
Laminar Flow-based Assays
Immunology
Leukocyte Adhesion
Tumor Cell Adhesion
Platelet-rich Plasma
Platelet-poor Plasma
Platelet Buffer
Hydrochloric Acid Ethanol
Rat Collagen Type One
Phosphate Buffered Saline
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