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Journal
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Immunology and Infection
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培養血管細胞と付着性血小板白血球動員を調査する層流に基づく試金
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
Please note that all translations are automatically generated.
Click here for the English version.
培養血管細胞と付着性血小板白血球動員を調査する層流に基づく試金
DOI:
10.3791/57009-v
•
08:50 min
•
April 09, 2018
•
Tanja Vajen
,
Alexandra C.A. Heinzmann
,
Annemiek Dickhout
,
Zhen Zhao
,
Magdolna Nagy
,
Johan W.M. Heemskerk
,
Rory R. Koenen
1
Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM)
,
Maastricht University
,
2
Department of Vascular Surgery, Shanghai Ninth People’s Hospital
,
Shanghai Jiao Tong University, School of Medicine
Chapters
00:05
Title
00:57
Flow-based Assay with Human Cells
02:28
Preparation of Adherent Platelet and Vascular Cell Monolayers
03:56
Fluorescent Labeling and Perfusion of Leukocytes
06:32
Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
08:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
炎症時に血管壁の損傷後、白血球は血管細胞と血小板との対話熱心。ここでは、白血球や細胞パートナー間の相互作用の根底にある分子メカニズムを特徴付ける簡単流アッセイについて述べる。
Tags
Keyword Extraction:Leukocyte Recruitment
Adherent Vascular Cells
Adherent Platelets
Flow Conditions
Laminar Flow-based Assays
Immunology
Leukocyte Adhesion
Tumor Cell Adhesion
Platelet-rich Plasma
Platelet-poor Plasma
Platelet Buffer
Hydrochloric Acid Ethanol
Rat Collagen Type One
Phosphate Buffered Saline
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