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Laminær strømning-baserte analyser undersøke leukocytter rekruttering på kulturperler vaskulære celler og tilhenger blodplater
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
DOI:

08:50 min

April 09, 2018

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Chapters

  • 00:05Title
  • 00:57Flow-based Assay with Human Cells
  • 02:28Preparation of Adherent Platelet and Vascular Cell Monolayers
  • 03:56Fluorescent Labeling and Perfusion of Leukocytes
  • 06:32Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
  • 08:09Conclusion

Summary

Automatic Translation

Leukocytter samhandle ivrig med vaskulære celler og blodplater etter fartøyet veggen skade eller under betennelse. Her beskriver vi en enkel laminær strømning-baserte analysen betegner molekylære mekanismer underlie samspillet mellom leukocytter og deres cellular partnere.

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