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Journal / Genetics / Kromatin Immunoprecipitation (ChIP) af Histon…
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JoVE Journal Genetics
Kromatin Immunoprecipitation (ChIP) af Histon ændringer fra Saccharomyces cerevisiae
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Kromatin Immunoprecipitation (ChIP) af Histon ændringer fra Saccharomyces cerevisiae

Article DOI: 10.3791/57080-v • 11:07 min • December 29th, 2017
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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Her, beskriver vi en protokol for kromatin immunoprecipitation af modificerede histoner fra spirende gær Saccharomyces cerevisiae. Immunoprecipitated DNA er efterfølgende anvendes til kvantitativ PCR afhøre overflod og lokalisering af Histon posttranslationelle ændringer i genomet.

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Genetik spørgsmålet 130 Epigenetik kromatin Histon ændringer methylering acetylation kromatin immunoprecipitation gær
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