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Genetics

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JoVE Journal Genetics
Kromatin Immunoprecipitation (ChIP) af Histon ændringer fra Saccharomyces cerevisiae
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Kromatin Immunoprecipitation (ChIP) af Histon ændringer fra Saccharomyces cerevisiae

Article DOI: 10.3791/57080
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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December 29th, 2017

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Her, beskriver vi en protokol for kromatin immunoprecipitation af modificerede histoner fra spirende gær Saccharomyces cerevisiae. Immunoprecipitated DNA er efterfølgende anvendes til kvantitativ PCR afhøre overflod og lokalisering af Histon posttranslationelle ændringer i genomet.

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