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Chapters
- 00:05Title
- 00:52In Vivo Crosslinking of Proteins to DNA
- 02:19Making Yeast Lysates
- 04:08Immunoprecipitation (IP) of Modified Histones
- 05:11Washing IPs, Eluting Histone-DNA Complexes and Reversing Protein-DNA Crosslinks
- 07:24Quantitative PCR (qPCR) to Detect Enriched Genomic Regions
- 08:46Results: ChIP Reveals Histone Modification Status at ant Genomic Region
- 10:17Conclusion
Her, beskriver vi en protokol for kromatin immunoprecipitation af modificerede histoner fra spirende gær Saccharomyces cerevisiae. Immunoprecipitated DNA er efterfølgende anvendes til kvantitativ PCR afhøre overflod og lokalisering af Histon posttranslationelle ændringer i genomet.
