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Genetics

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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) Histone endringer fra Saccharomyces cerevisiae
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Journal (Genetics)
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
Journal (Genetics)
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
 
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Chromatin Immunoprecipitation (ChIP) Histone endringer fra Saccharomyces cerevisiae

Article DOI: 10.3791/57080
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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December 29th, 2017

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Her beskriver vi en protokoll for chromatin immunoprecipitation av modifisert histones fra spirende gjær Saccharomyces cerevisiae. Immunoprecipitated DNA blir brukt i kvantitative PCR avhøre overflod og lokalisering av histone post-translasjonell endringer i genomet.

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