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Dissekera multi signalering proteinkomplex av Bimolecular komplettering affinitet rening (BiCAP)
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
DOI:

06:45 min

June 15, 2018

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Chapters

  • 00:04Title
  • 00:45Plasmid Cloning
  • 02:54Cell Culture and Transfection
  • 03:41Harvesting Lysates
  • 04:31Affinity Purification and Preparation of BiCAP Eluant for Western Blotting
  • 05:22Results: BiCAP Method is a Powerful Technique to Specifically Purify and Characterize Interacting Proteins
  • 06:06Conclusion

Summary

Automatic Translation

Detta manuskript beskriver protokollet för Bimolecular komplettering affinitet rening (BiCAP). Denna nya metod underlättar specifika isolering och nedströms proteomiska karakterisering av några två samverkande proteiner, medan exklusive FN-komplex enskilda proteiner samt komplex bildas med konkurrerande bindande partner.

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