Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Dissezione multiproteici segnalazione di…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Dissezione multiproteici segnalazione di purificazione di affinità di complementazione bimolecolare (BiCAP)
Peptide-based Identification of Functional Motifs and their Binding Partners
Journal (Immunology and Infection)
Peptide-based Identification of Functional Motifs and their Binding Partners
Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
Journal (Immunology and Infection)
Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
 
Click here for the English version

Dissezione multiproteici segnalazione di purificazione di affinità di complementazione bimolecolare (BiCAP)

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Questo manoscritto descrive il protocollo per purificazione di affinità complementazione bimolecolare (BiCAP). Questo metodo novello facilita l'isolamento specifico e caratterizzazione proteomica a valle di qualsiasi due proteine interagenti, mentre escluse ONU-complessato singole proteine, nonché complessi formati con concorrenti partner di legame.

Tags

Immunologia e infezione problema 136 interazioni proteina-proteina proteomica interattomica BiCAP trasduzione del segnale complementazione bimolecolare fluorescenza Nanobody
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter