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To-trins tilgang til at udforske tidlige - og sene-stadier af orgel dannelse i den aviær Model: Thymus og Biskjoldbruskkirtlerne Organogenesis paradigme
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm
DOI:

13:43 min

June 17, 2018

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Chapters

  • 00:00Title
  • 03:05Isolation of Quail Embryonic Region Containing the Presumptive Territory of Thymic and Parathyroids Rudiments
  • 05:49Organotypic Assay: In Vitro Culture of the Embryonic Region Containing the Presumptive Territory of Thymic and Parathyroids Rudiments
  • 08:17Chorioallantoic Membrane (CAM) Assay: Grafting of Cultured Explants onto CAM, Ectopic Organ Formation after 10 Days in CAM
  • 10:59Representative Results of the Two-Step Approach
  • 13:08Conclusion

Summary

Automatic Translation

Denne artikel indeholder en opdateret tilgang til den klassiske vagtler-kylling chimera system at studere orgel dannelse, ved at kombinere roman i in vitro og i ovo eksperimentelle procedurer.

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