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Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue
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JoVE Journal Neuroscience
Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

DOI:
10.3791/57123-v

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06:14 min

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May 08, 2018

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, , , ,
1Department of Neurosurgery, Gangnam Severance Hospital,Yonsei University College of Medicine, 2Brain Korea 21 PLUS Project for Medical Science,Yonsei University, 3The Spine and Spinal Cord Institute, Biomedical Center, Gangnam Severance Hospital,Yonsei University College of Medicine, 4Columbia University College of Physicians and Surgeons

Chapters

  • 00:05Title
  • 01:12PACT (Passive Cleaning Technique)
  • 02:49psPACT (process separate Passive Clearing Technique)
  • 04:24mPACT (modified Passive Clearing Technique)
  • 05:02Results: Comparison of mPACT to Other Methods
  • 05:35Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present two novel methodologies, psPACT and mPACT, for achieving maximal optical transparency and subsequent microscopic analysis of tissue vasculature in the intact rodent whole CNS.

Tags

Keywords: Optical TransparencyPassive ClearingRapid ProductionPsPACTMPACTAlzheimer’s DiseaseInjuryDegenerationTumorigenesisPACTVA-044HydrogelClearing Solution

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