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以乌苏图病毒为例，验证全基因组纳米孔测序
JoVE Journal
Genetics
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JoVE Journal Genetics
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
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以乌苏图病毒为例，验证全基因组纳米孔测序

DOI:
10.3791/60906-v

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05:45 min

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March 11, 2020

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, , ,
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Chapters

  • 00:04Introduction
  • 00:59Nanopore Data Consensus Sequence Generation
  • 02:33Nanopore Sequencing Error Profile Compensation
  • 03:54Results: Representative Nanopore Sequencing Error Rate Overview
  • 04:58Conclusion

Summary

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我们之前在纳米孔测序平台上验证了基于安普利子全基因组Usutu病毒（USUV）测序的协议。在这里，我们更详细地描述了使用的方法，并确定纳米孔R10流动单元的误差率。

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Artificial IntelligenceAI Writing AssistantsCopywritingContent GenerationText-based ContentContent CreationAI-assisted WritingWriter’s BlockContent EfficiencyReal-time SequencingVirus Genetic SequencingNanopore SequencingUsutu VirusError RateRead CoverageSequence DataConsensus SequenceReference-based AlignmentMinimap2SamtoolsKMA

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