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Lipid indeks fastsettelse av flytende fluorescens utvinning i Fungal patogen Ustilago Maydis
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
DOI:

10:22 min

April 03, 2018

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Chapters

  • 00:04Title
  • 01:07Culture Condition and Cell Fixation
  • 04:14Liquid Fluorescence Recovery Assay (LFR)
  • 06:13Calculations of the LD Index
  • 07:26Results: LD Index Measurements Enable the Study of TAG Dynamics in U. maydis
  • 08:59Conclusion

Summary

Automatic Translation

Her beskriver vi en protokoll for å få lipid slippverktøy indeksen (LD index) for å studere dynamikken i triacylglycerols i cellene kulturperler høy gjennomstrømming eksperimenter. LD indeks analysen er en enkel og pålitelig metode som bruker BODIPY 493/503. Denne analysen trenger ikke dispendious lipid utvinning eller mikroskopi analyse.

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