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小鼠小肠免疫系统淋巴细胞的分离
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolating Lymphocytes from the Mouse Small Intestinal Immune System
DOI:

11:28 min

February 28, 2018

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Chapters

  • 00:04Title
  • 01:00Isolation of the Mesenteric Lymph Nodes, Intestines, and Peyer’s Patches
  • 03:45Isolation of Lymphocytes from the Inductive Sites
  • 04:22Isolation of Intraepithelial Lymphocytes from the Small Intestine
  • 07:28Isolation of Lamina Propria Lymphocytes from the Small Intestine
  • 09:39Results: Characterization of Isolated Lymphocytes
  • 10:52Conclusion

Summary

Automatic Translation

在这里, 我们描述了一个详细的协议, 从诱导部位分离淋巴细胞, 包括肠道相关淋巴组织 Peyer 的补丁和引流肠系膜淋巴结, 和效应部位, 包括固有层和小肠上皮的小肠免疫系统。

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