A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 01:00Isolation of the Mesenteric Lymph Nodes, Intestines, and Peyer’s Patches
- 03:45Isolation of Lymphocytes from the Inductive Sites
- 04:22Isolation of Intraepithelial Lymphocytes from the Small Intestine
- 07:28Isolation of Lamina Propria Lymphocytes from the Small Intestine
- 09:39Results: Characterization of Isolated Lymphocytes
- 10:52Conclusion
Her beskriver vi en detaljeret protokol til isolering af lymfocytter fra de induktive websteder inklusive de Peyerske plaques gut-associerede lymfoide væv og afdrypning mesenteriallymfeknuderne lymfeknuder, og effektor herunder lamina propria og den intestinale epitel af lille tarm immunsystemet.
