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Méthode quantitative et Qualitative de sphingomyéline par LC-MS à l’aide de deux Stable isotopiquement étiquetés sphingomyéline espèces
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
DOI:

08:53 min

May 07, 2018

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Chapters

  • 00:05Title
  • 00:33Preparation of Lipid Samples
  • 03:06Sample Preparation for Calibration Curve and Method Validation
  • 03:50SM Analysis by LC-ESI-MS/MS
  • 05:33Batch File Creation and Assigning the MS Three Product Ion Spectra of Sphingomyelin
  • 06:23Quantitative Analysis of SM by LC-ESI-MS/MS
  • 07:02Results: Validating the Quantitative and Qualitative Method for Sphingomyelin
  • 08:01Conclusion

Summary

Automatic Translation

Ici, nous présentons un protocole pour quantifier et qualifier chaque espèce de sphingomyéline multiple réaction sous contrôle et le mode MS/MS/MS, respectivement.

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