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Belysning op veje til Caspase aktivering ved hjælp af Bimolecular fluorescens komplementering
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
DOI:

08:47 min

March 05, 2018

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Chapters

  • 00:04Title
  • 00:36Transfection of Cells to Introduce the Caspase Bimolecular Fluorescence Complementation (BiFC) Components
  • 02:42Three-dimensional Imaging of Caspase BiFC
  • 05:00Time-lapse Imaging of Caspase BiFC
  • 06:19Results: Visualization of Caspase Activation Pathways Using Caspase Bimolecular Fluorescence Complementation
  • 07:37Conclusion

Summary

Automatic Translation

Denne protokol beskriver caspase Bimolecular fluorescens komplementering (BiFC); en billeddannelse-baserede metode, der kan bruges til at visualisere induceret nærhed af initiatoren caspases, som er det første skridt i deres aktivering.

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