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应用流式细胞仪对人体脂肪组织免疫细胞的表征
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
应用流式细胞仪对人体脂肪组织免疫细胞的表征
DOI:
10.3791/57319-v
•
07:32 min
•
March 06, 2018
•
Suzan Wetzels*
1,2,3
,
Mitchell Bijnen*
1,2
,
Erwin Wijnands
4
,
Erik A.L. Biessen
4
,
Casper G. Schalkwijk
2
,
Kristiaan Wouters
2
1
Department of Internal Medicine
,
MUMC
,
2
CARIM
,
MUMC
,
3
Department of Immunology and Biochemistry, Biomedical Research Institute
,
Hasselt University
,
4
Department of Pathology
,
MUMC
Chapters
00:04
Title
01:06
Stromal Vascular Fraction (SVF) Adipose Tissue (AT) Isolation
02:42
SVF Staining and Flow Cytometry Analysis
04:50
Results: Representative Visceral AT Immune Cell Abundance
06:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种用流式细胞仪从脂肪组织中分离免疫细胞来分析脂肪组织免疫细胞含量的方法和后续分析。
Tags
Flow Cytometry
Stromal Vascular Fraction
Immune Cells
Phenotyping
Metabolic Research
Obesity
Inflammation
Cell Isolation
Cell Staining
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