Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

Patrick Haubruck; Aimee  C. Colbath; Yolanda Liu; Shihani Stoner; Cindy Shu; Christopher  B. Little

doi:10.3791/61008

/

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

19,031 Views

•

12:23 min

•

April 24, 2020

DOI:

10.3791/61008-v

DOI:

10.3791/61008-v

•

12:23 min

April 24, 2020

•

74 Views

Patrick Haubruck^1,2, Aimee C. Colbath^1,3, Yolanda Liu¹, Shihani Stoner¹, Cindy Shu¹, Christopher B. Little¹

¹Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital,University of Sydney, ²HTRG – Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery,Heidelberg University Hospital, ³Department of Large Animal Clinical Sciences, College of Veterinary Medicine,Michigan State University

Summary

Here, we describe a detailed and reproducible flow cytometry protocol to identify monocyte/macrophage and T-cell subsets using both extra- and intracellular staining assays within the murine spleen, bone marrow, lymph nodes and synovial tissue, utilizing an established surgical model of murine osteoarthritis.