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Chapters
- 00:04Title
- 00:45Immobilization of the Receptor (Integrin α2A-domain) to a Microtiter Plate
- 02:09Preparation of a Serial Dilution Row of the Ligand (Rhodocetin)
- 03:25Binding of Ligand at Different Concentrations to Immobilized Receptor
- 05:03Quantification of Receptor-bound Ligand by ELISA
- 07:06Evaluation of the Titration Signals
- 09:30Results: Rhodocetin Binds with Lower Affinity to Mutant Integrin α2A-domain
- 10:58Conclusion
