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Titrering ELISA som en metode til at bestemme dissociationskonstant Receptor Ligand interaktion
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
DOI:

12:38 min

February 15, 2018

Chapters

  • 00:04Title
  • 00:45Immobilization of the Receptor (Integrin α2A-domain) to a Microtiter Plate
  • 02:09Preparation of a Serial Dilution Row of the Ligand (Rhodocetin)
  • 03:25Binding of Ligand at Different Concentrations to Immobilized Receptor
  • 05:03Quantification of Receptor-bound Ligand by ELISA
  • 07:06Evaluation of the Titration Signals
  • 09:30Results: Rhodocetin Binds with Lower Affinity to Mutant Integrin α2A-domain
  • 10:58Conclusion

Summary

Automatic Translation

En detaljeret protokol til at udføre en titrering ELISA er beskrevet. Derudover præsenteres en roman algoritme at evaluere titrering ringprøver og opnå en dissociationskonstant af bindingen af et opløseligt ligand til en mikrotiter plade-immobiliseret receptor.

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