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Titrering ELISA som metode for å bestemme dissosiasjon konstant reseptor Ligand interaksjon
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
DOI:

12:38 min

February 15, 2018

Chapters

  • 00:04Title
  • 00:45Immobilization of the Receptor (Integrin α2A-domain) to a Microtiter Plate
  • 02:09Preparation of a Serial Dilution Row of the Ligand (Rhodocetin)
  • 03:25Binding of Ligand at Different Concentrations to Immobilized Receptor
  • 05:03Quantification of Receptor-bound Ligand by ELISA
  • 07:06Evaluation of the Titration Signals
  • 09:30Results: Rhodocetin Binds with Lower Affinity to Mutant Integrin α2A-domain
  • 10:58Conclusion

Summary

Automatic Translation

En detaljert protokoll for å utføre en titrering ELISA er beskrevet. Dessuten vises en ny algoritme evaluere titrering ELISAs og hente en dissosiasjon konstant av binding av en løselig ligand til en være ferdig innen plate-immobilisert reseptor.

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