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Bioengineering
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原发性肾组织段的显微解剖与新型无支架结构技术的结合
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology
Please note that all translations are automatically generated.
Click here for the English version.
原发性肾组织段的显微解剖与新型无支架结构技术的结合
DOI:
10.3791/57358-v
•
09:00 min
•
March 27, 2018
•
Chase A. Arbra*
1
,
Satish N. Nadig*
1
,
Sarah Grace Dennis
,
Sanket Pattanaik
,
Heather A. Bainbridge
,
J. Matthew Rhett
,
Stephen A. Fann
,
Carl Atkinson
,
Michael J. Yost
1
Department of Surgery
,
Medical University of South Carolina
Chapters
00:04
Title
00:54
Surgical Preparations
02:28
Murine Nephrectomy
04:05
Murine Kidney Microdissection for Renal Segment Isolation
07:00
Results: Characterizing the Renal Segments
08:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
组织工程的肾脏结构提供了解决的器官短缺和有害影响的透析。在这里, 我们描述了一个小鼠肾脏的显微解剖的协议, 以隔离皮质髓质段。这些片段植入无支架细胞结构, 形成肾 organoids。
Tags
Microdissection
Scaffold-free Construct
End-stage Renal Disease
Sterile Technique
Mouse Model
Anesthesia
Hair Removal
Skin Sterilization
Surgical Procedure
Peritoneum
Abdominal Cavity
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