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Microdissection af primær Renal væv segmenter og iblandingen med romanen stillads-fri konstruktion teknologi
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology
DOI:

09:00 min

March 27, 2018

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Chapters

  • 00:04Title
  • 00:54Surgical Preparations
  • 02:28Murine Nephrectomy
  • 04:05Murine Kidney Microdissection for Renal Segment Isolation
  • 07:00Results: Characterizing the Renal Segments
  • 08:10Conclusion

Summary

Automatic Translation

Væv-manipuleret renal konstruktioner giver en løsning til organmangel og skadelige virkninger af dialyse. Her, vi beskriver en protokol til micro dissekere murine nyrer til isolation af cortico-medullær segmenter. Disse segmenter er implanteret i stillads-fri cellulære konstruktioner, danner renale organoids.

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