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Mikromanipulation von Chromosomen in Insekten Spermatozyten
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Micromanipulation of Chromosomes in Insect Spermatocytes
DOI:

05:45 min

October 22, 2018

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Chapters

  • 00:04Title
  • 00:34Preparation of Primary Insect Spermatocyte Cell Culture for Micromanipulation
  • 01:11Spermatocyte Culture Preparation for Viewing on Microscope
  • 02:03Production of the Microneedle
  • 02:42Positioning the Micromanipulator
  • 04:00Micromanipulation of Chromosomes
  • 04:43Results: Chromosomes Can be Detached from Spindle in Prophase, Metaphase, and Anaphase
  • 05:07Conclusion

Summary

Automatic Translation

In diesem Protokoll beschreiben wir die Auswahl und Vorbereitung der entsprechenden Zellen für Mikromanipulation und den Einsatz von piezoelektrischen Mikromanipulator Chromosomen in den Zellen neu zu positionieren.

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