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Micromanipolazione dei cromosomi in insetto spermatociti
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Micromanipulation of Chromosomes in Insect Spermatocytes
DOI:

05:45 min

October 22, 2018

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Chapters

  • 00:04Title
  • 00:34Preparation of Primary Insect Spermatocyte Cell Culture for Micromanipulation
  • 01:11Spermatocyte Culture Preparation for Viewing on Microscope
  • 02:03Production of the Microneedle
  • 02:42Positioning the Micromanipulator
  • 04:00Micromanipulation of Chromosomes
  • 04:43Results: Chromosomes Can be Detached from Spindle in Prophase, Metaphase, and Anaphase
  • 05:07Conclusion

Summary

Automatic Translation

In questo protocollo, descriviamo la selezione e la preparazione delle celle appropriate per micromanipolazione e l'uso di un micromanipolatore piezoelettrico per riposizionare i cromosomi all'interno di quelle cellule.

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