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Hurtig In Vivo evaluering af Adjuverendes cytotoksiske T-lymfocytter Generation kapaciteter for Vaccine udvikling
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JoVE Journal Medicine
Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Hurtig In Vivo evaluering af Adjuverendes cytotoksiske T-lymfocytter Generation kapaciteter for Vaccine udvikling

DOI:
10.3791/57401-v

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09:03 min

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June 19, 2018

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, , , , , , ,
1Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research

Chapters

  • 00:05Title
  • 01:03OT-I T Cell Staining and Adoptive Transfer
  • 04:22Endo-Free OVA +/- Adjuvant Immunization and Lymphocyte Isolation
  • 05:21Flow Cytometry
  • 07:30Results: Representative Adjuvant Drive Cytotoxic T Cell Generation
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her en ansøgning om en standard immunologiske teknik (CFSE farves OT-jeg spredning) skal hurtigt overvåge adjuvans-medieret cytotoksiske T-lymfocytter (CTL) generation in vivo. Denne hurtig estimering af CTL kapacitet er nyttige for udviklingen af forebyggende vacciner mod intracellulære patogener samt terapeutiske kræft vacciner.

Tags

OT1 CD8 T CellsCFSEAdoptive TransferAdjuvantCytotoxic T Lymphocyte (CTL) ResponseVaccine DevelopmentLymph NodeSpleenMagnetic Bead Isolation

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