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Isolering af primære humane Decidual celler fra de føtale membraner af udtrykket efterbyrd
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
DOI:

07:37 min

April 30, 2018

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Chapters

  • 00:05Title
  • 00:42Collection of Decidual Tissue from Term Placental Membranes
  • 01:57Washing and Enzymatic Digestion of the Decidual Tissue
  • 03:56Generation of Single Cell Suspension
  • 05:22Results: Use of Flow Cytometry and Immunocytochemistry to Isolate the Primary Human Decidual Cells
  • 07:11Conclusion

Summary

Automatic Translation

Denne protokol viser en metode til isolering af primær decidual menneskeceller indsamlet fra de føtale membraner i sigt efterbyrd, som kan bruges til en lang række applikationer (dvs. immuncytokemi, flowcytometri, etc.) med det formål at studere rollen, som forskellige cellepopulationer i graviditetskomplikationer.

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