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Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions
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Neuroscience
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JoVE Journal Neuroscience
Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

DOI:
10.3791/57482-v

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10:45 min

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May 05, 2018

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, , ,
1Department of Biological Sciences,Wright State University

Chapters

  • 00:04Title
  • 01:18Levator Auris Longus Muscle Isolation
  • 05:22Isolation of Nerve
  • 06:50Electrophysiology Experiment
  • 08:00Identification of Neuromuscular Junction using Fluorescence
  • 08:54Results: Current-clamp and Voltage-clamp Recording of Membrane Properties
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol described in this paper uses the mouse levator auris longus (LAL) muscle to record spontaneous and nerve-evoked postsynaptic potentials (current-clamp) and currents (voltage-clamp) at the neuromuscular junction. Use of this technique can provide key insights into mechanisms of synaptic transmission under normal and disease conditions.

Tags

Levator Auris LongusNeuromuscular TransmissionVoltage ClampSynaptic CurrentsEnd Plate CurrentQuantal ContentProbability Of ReleaseNeurotransmissionMuscle ExcitabilitySynaptic FunctionNerve-muscle PreparationMammalianDissectionElectrophysiology

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