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提耳伸肌压钳条件下哺乳动物神经肌肉传输的检查准备
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JoVE Journal Neuroscience
Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions
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提耳伸肌压钳条件下哺乳动物神经肌肉传输的检查准备

DOI:
10.3791/57482-v

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10:45 min

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May 05, 2018

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, , ,
1Department of Biological Sciences,Wright State University

Chapters

  • 00:04Title
  • 01:18Levator Auris Longus Muscle Isolation
  • 05:22Isolation of Nerve
  • 06:50Electrophysiology Experiment
  • 08:00Identification of Neuromuscular Junction using Fluorescence
  • 08:54Results: Current-clamp and Voltage-clamp Recording of Membrane Properties
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

本文所描述的协议使用鼠标提耳肌 (拉尔) 肌肉记录自发性和神经诱发突触后电位 (电流钳) 和电流 (电压钳) 在神经肌肉连接。使用该技术可以为正常和疾病条件下突触传递机制提供关键的见解。

Tags

Levator Auris LongusNeuromuscular TransmissionVoltage ClampSynaptic CurrentsEnd Plate CurrentQuantal ContentProbability Of ReleaseNeurotransmissionMuscle ExcitabilitySynaptic FunctionNerve-muscle PreparationMammalianDissectionElectrophysiology

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