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Ablation af Neuronal indbyggere ved hjælp af en to-foton Laser og dets vurdering ved hjælp af Calcium Imaging og adfærdsmæssige optagelse i zebrafisk larver
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
DOI:

10:29 min

June 02, 2018

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Chapters

  • 00:04Title
  • 00:51Ablation of a Subpopulation of Neurons Using a Two-photon Laser Microscope
  • 04:47Calcium imaging to Record Prey-Evoked Neuronal Activity in the Pretectum-Ablated Zebrafish Larvae
  • 06:38Assessment of Behavioral Consequences Following Laser Ablation
  • 07:30Results: Assessment of Laser Ablated Pretectal Neurons with Calcium Imaging
  • 09:05Conclusion

Summary

Automatic Translation

Vi præsenterer her, en protokol til at ablate en genetisk mærket delpopulation af neuroner ved hjælp af en to-foton laser fra zebrafisk larver.

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