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Ablasyon nöronal nüfusunun iki fotonlu lazer ve kalsiyum görüntüleme ve davranış kayıt zebra balığı larva kullanarak kendi değerlendirme kullanma
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
DOI:

10:29 min

June 02, 2018

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Chapters

  • 00:04Title
  • 00:51Ablation of a Subpopulation of Neurons Using a Two-photon Laser Microscope
  • 04:47Calcium imaging to Record Prey-Evoked Neuronal Activity in the Pretectum-Ablated Zebrafish Larvae
  • 06:38Assessment of Behavioral Consequences Following Laser Ablation
  • 07:30Results: Assessment of Laser Ablated Pretectal Neurons with Calcium Imaging
  • 09:05Conclusion

Summary

Automatic Translation

Burada, zebra balığı larvaları üzerinden bir iki fotonlu lazer nöronların genetik olarak etiketlenmiş bir subpopulation ablate için bir protokol mevcut.

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