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球体、菌落形成测定和斑马鱼移植对抗癌香豆素 OT48 生物活性的临床前评价
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JoVE Journal
Cancer Research
Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts
Please note that all translations are automatically generated.
Click here for the English version.
球体、菌落形成测定和斑马鱼移植对抗癌香豆素 OT48 生物活性的临床前评价
DOI:
10.3791/57490-v
•
09:20 min
•
June 26, 2018
•
Jin-Young Lee*
1
,
Aloran Mazumder*
1
,
Marc Diederich
1
Department of Pharmacy, College of Pharmacy
,
Seoul National University
Chapters
00:04
Title
00:33
Colony Formation Assays
03:47
Zebrafish Egg Production and Incubation for Xenograft Assay
05:05
Compound-treated Cell Preparation and Microinjection of Cancer Cells for Xenograft Formation
07:21
Results: Inhibitory Potential of Anticancer Compounds on Spheroid Formation and
In Vivo
Tumor Formation in Zebrafish
08:29
Conclusion
Summary
Automatic Translation
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在这里, 我们提出了应用3D 培养和斑马鱼的抗癌香豆素的临床前筛选。
Tags
Preclinical Assessment
Bioactivity
Anticancer Coumarin OT48
Spheroids
Colony Formation Assays
Zebrafish Xenografts
In Vitro
In Vivo
3D Culture Systems
A549 Cells
RPMI 1640 Medium
Methylcellulose-based Medium
MTT Assay
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