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Immunology and Infection
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整合影像流式细胞仪和转录组分析, 评估改变的内吞 CD1d 贩运
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
Please note that all translations are automatically generated.
Click here for the English version.
整合影像流式细胞仪和转录组分析, 评估改变的内吞 CD1d 贩运
DOI:
10.3791/57528-v
•
09:01 min
•
October 29, 2018
•
Manju Sharma
,
Xiang Zhang
,
Shouxiong Huang
1
Department of Environmental Health
,
University of Cincinnati College of Medicine
Chapters
00:04
Title
01:14
Pollutant-exposed Human Monocyte-derived Dendritic Cell (DC) Sequencing
04:10
Imaging Flow Cytometry Analysis
05:42
Results: CD1d and Lamp1 Co-localization Analysis
07:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
成像流式细胞术提供了一种理想的方法来检测细胞在个体和种群水平的形态学和功能改变。对污染物暴露的人树突状细胞中脂质抗原表达的破坏内吞功能进行了转录组基因表达和蛋白质贩运形态表现的综合分析。
Tags
Imaging Flow Cytometry
Transcriptomic Profiling
CD1d Trafficking
Next-generation Sequencing
Protein Function
Dendritic Cells
Sequencing Workflow
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